Coding
Rci

Part:BBa_K361000:Design

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM10_Hong_Kong-CUHK   (2010-10-24)


Rci site-specific recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 362
    Illegal NgoMIV site found at 857
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene is come from muliple inversion systems in R64 plasmid and there is no further modification. Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process.


Source

It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1

References